NMT是基因功能的活体检测技术，已被31位诺贝尔奖得主所在单位，及北大、清华、中科院使用。
期刊：Tree Physiology
主题：红柳和棉花在干旱胁迫下对硝吸收的差异
标题：Characterization and comparison of nitrate fluxes in Tamarix ramosissima and cotton roots under simulated drought conditions
影响因子：3.389
检测指标：NO3-流速
检测样品：红柳、棉花根部（距离根尖5mm，20mm）
NO3-流速流实验处理方法：
15% PEG瞬时处理
NO3-流速流实验测试液成份：
0.1 mM KNO3, 0.1 mM MgCl2, 0.1 mM NaCl, 0.1 mM CaCl2 and 0.3 mM 2-(N-morpholino) ethanesulfonic acid hydrate (MES), pH 6.2
作者：中科院南京土壤所施卫明、李光杰、张琳
英文摘要
Tamarix ramosissima Ledeb., a major host plant for the parasitic angiosperm Cistanche tubulosa, and known for its unique drought tolerance, has significant ecological and economic benefits. However, the mechanisms of nitrogen acquisition by the T. ramosissima root system under drought have remained uncharacterized.
Here, uptake of nitrate (NO3−) in various regions of the root system was measured in T. ramosissima using Non-invasive Micro-test Technology at the cellular level, and using a 15NO3–-enrichment technique at the whole-root level. These results were compared with responses in the model system cotton (Gossypium hirsutum L.). Tamarix ramosissima had lower net NO3– influx and a significantly lower Km (the apparent Michalis–Menten constant; 8.5 μM) for NO3– uptake than cotton under normal conditions.
Upon simulated drought conditions, using polyethylene glycol (PEG), NO3– flux in cotton switched from net influx to net efflux, with a substantive peak in the white zone (WZ) of the root. There were no significant NO3– influx signals observed in the WZ of T. ramosissima under control conditions, whereas PEG treatment significantly enhanced NO3– influx in the WZ of T. ramosissima. The effect of PEG application on NO3– fluxes was highly localized, and the increase in net NO3– influx in response to PEG stimulation was also found in C. tubulosa-inoculated T. ramosissima. Consistently, root nitrogen (N) content and root biomass were higher in T. ramosissima than in cotton under PEG treatment.
Our study provides insights into NO3– uptake and the influence of C. tubulosa inoculation in T. ramosissima roots during acclimation to PEG-induced drought stress and provides guidelines for silvicultural practice and for breeding of T. ramosissima under coupled conditions of soil drought and N deficiency.
中文摘要（谷歌机翻）
Tamarix ramosissima Ledeb。是寄生被子植物肉stan蓉的主要寄主植物，以其独特的耐旱性而闻名，具有显着的生态和经济效益。然而，在干旱条件下，T。ramosissima根系对氮素的吸收机制尚未阐明。
在这里，使用非侵入性微测试技术在细胞水平上使用毛细线虫，并在全根水平上使用15NO3-富集技术测量了毛滴虫在根系各个区域中**盐（NO3-）的吸收。将这些结果与模型系统棉（Gossypium hirsutum L.）中的响应进行比较。在正常条件下，柳的净NO3-流入量较低，而NO3-吸收量的Km（表观Michalis-Menten常数； 8.5μM）则显着低于棉花。
在模拟干旱条件下，使用聚乙二醇（PEG），棉花中的NO3-通量从净流入量转换为净流出量，在根部的白色区域（WZ）出现一个实质性的峰值。在控制条件下，没有观察到明显的NO3-流入信号，而PEG处理显着增强了T. ramosissima的W3区NO3-流入。 PEG施用对NO3-通量的影响高度局限，并且在接种C.tubulosa的T. ramosissima中还发现了响应PEG刺激的NO3-净流入量的增加。一致地，在PEG处理下，T.ramosissima的根氮（N）含量和根生物量均高于棉花。
我们的研究为适应PEG诱导的干旱胁迫提供了NO3的吸收以及在T. ramosissima根中接种C.tubulosa的影响，并为土壤干旱和N耦合条件下的营林实践和T. ramosissima育种提供了指导。不足。
结果表明：使用PEG进行瞬时处理后，棉花根系的NO3-由吸收转为外排，最高值可达400 pmol·m-2·s-1，由此降低根系的N含量；而红柳根系经PEG瞬时处理后吸收明显增加，最高可达300 pmol·m-2·s-1，提高了根系的N含量。
文章链接：
https://academic.oup.com/treephys/article-abstract/39/4/628/5253231?redirectedFrom=fulltext