NMT历史上的今天
2017年11月07日，首都师范大学印莉萍、谭松利用NMT在Traffic上发表了标题为TMD1 domain and CRAC motif determine the association and disassociation of MxIRT1 with detergent‐resistant membranes
的研究成果。
期刊：Traffic
主题：TMD1域和CRAC基序决定MxIRT1与耐去污剂膜的缔合和解离
标题：TMD1 domain and CRAC motif determine the association and disassociation of MxIRT1 with detergent‐resistant membranes
影响因子：4.133
检测指标：Cd2+流速
作者：首都师范大学印莉萍、谭松
英文摘要
Iron is essential for most living organisms. The iron‐regulated transporter1 (IRT1) plays a major role in iron uptake in roots, and its trafficking from endoplasmic reticulum (ER) to plasma membrane (PM) is tightly coordinated with changes in iron environment.
However, studies on the IRT1 response are limited. Here, we report that Malus xiaojinesis IRT1 (MxIRT1) associates with detergent‐resistant membranes (DRMs, a biochemical counterpart of PM microdomains), whereas the PM microdomains are known platforms for signal transduction in the PM. Depending on the shift of MxIRT1 from microdomains to homogeneous regions in PM, MxIRT1‐mediated iron absorption is activated by the cholesterol recognition/interaction amino acid consensus (CRAC) motif of MxIRT1.
MxIRT1 initially associates with DRMs in ER via its transmembrane domain 1 (TMD1), and thus begins DRMs‐dependent intracellular trafficking. Subsequently, MxIRT1 is sequestered in COPII vesicles via the ER export signal sequence in MxIRT1.
These studies suggest that iron homeostasis is influenced by the CRAC motif and TMD1 domain due to their determination of MxIRT1‐DRMs association.
中文摘要（谷歌机翻）
铁对大多数生物都是必不可少的。铁调节转运蛋白1（IRT1）在根部吸收铁中起主要作用，其从内质网（ER）到质膜（PM）的运输与铁环境的变化紧密相关。
但是，有关IRT1反应的研究有限。在这里，我们报告说，小金海棠IRT1（MxIRT1）与耐去污剂的膜（DRM，PM微域的生化对应物）相关，而PM微域是PM中信号转导的已知平台。取决于MxIRT1从PM的微区到均质区的转移，MxIRT1介导的铁吸收被MxIRT1的胆固醇识别/相互作用氨基酸共有（CRAC）基序激活。
MxIRT1最初通过其跨膜结构域1（TMD1）与ER中的DRM结合，从而开始依赖DRM的细胞内运输。随后，通过MxIRT1中的ER出口信号序列将MxIRT1隔离在COPII囊泡中。
这些研究表明，由于铁的动态平衡决定了MxIRT1-DRM的相关性，因此受CRAC基序和TMD1域的影响。
G) Average Cd2+flux in the different transgenic Arabidopsis root hairs in 7-minute incubation with 0.05 mM CdCl2 measured by Non-invasion Micro-test Technology. The bars represent the means standard errors of 3 independent analyses (n = 3). Significant differences were determined by
Student’s t test. *P < 0.05, **P < 0.01
文章链接： https://linkinghub.elsevier.com/retrieve/pii/S0167880918304377