Cd40lg (Mouse) Recombinant Protein
产品名称： Cd40lg (Mouse) Recombinant Protein
英文名称： Cd40lg (Mouse) Recombinant Protein
产品编号： P4590
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Mouse Cd40lg (P27548) recombinant protein expressed in Escherichia coli.
- Sequence:
- MQRGDEDPQIAAHVVSEANSNAASVLQWAKKGYYTMKSNLVMLENGKQLTVKREGLYYVYTQVTFCSNREPSSQRPFIVGLWLKPSSGSERILLKAANTHSSSQLCEQQSVHLGGVFELQAGASVFVNVTEASQVIHRVGFSSFGLLKL
- Theoretical MW (kDa):
- 16.4
- Form:
- Lyophilized
- Preparation Method:
- Escherichia coli expression system
- Endotoxin Level:
- < 0.01 ng/ug or < 0.1 EU/ug.
- Activity:
- The activity is determined by the dose-dependent production of IL-8 by human PBMCs. The expected ED50 for this effect is 3.7-5.5 ng/mL.
- Storage Buffer:
- Lyophilized with 10 mM Na2PO4, pH 7.5.
- Storage Instruction:
- Store at -20°C on dry atmosphere.
After reconstitution with sterilized water, store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- 1 ug/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditions
- Note:
- Result of activity analysis
-
Serial dilutions of mouse Cd40lg, starting at 250 ng/mL, were added to human PBMCs. After 39 hours, cell supernatant was collected and human IL-8 was measured via ELISA and the linear portion of the curve was us used to calculate the ED50.
- Application Image
- Functional Study
- SDS-PAGE